Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194655
Type: Artigo
Title: Fluoride effect on the activity of enamel matrix proteinases in vitro
Author: Gerlach, R. F.
de Souza, A. P.
Cury, J. A.
Line, S. R.
Abstract: Dental fluorosis is common in individuals exposed to different sources of fluoride during tooth development. The mechanism causing this enamel defect is still unknown. Enamel matrix proteinases play a central role in the maturation of dental enamel, and inhibition of these enzymes by fluoride has been one explanation for dental fluorosis. We have investigated the effect of fluoride on the activity of enamel matrix proteinases using a colorimetric assay, casein zymography, and an enamel protein degradation assay. Fluoride (625 microM to 10 mM) inhibited neither the enzymatic activity of the crude matrix extract nor the activity of individual enamel enzymes separated by SDS-PAGE. The proposition that fluoride could directly inhibit enzymes was not confirmed in this study.
Dental fluorosis is common in individuals exposed to different sources of fluoride during tooth development. The mechanism causing this enamel defect is still unknown. Enamel matrix proteinases play a central role in the maturation of dental enamel, and inhibition of these enzymes by fluoride has been one explanation for dental fluorosis. We have investigated the effect of fluoride on the activity of enamel matrix proteinases using a colorimetric assay, casein zymography, and an enamel protein degradation assay. Fluoride (625 μM to 10 mM) inhibited neither the enzymatic activity of the crude matrix extract nor the activity of individual enamel enzymes separated by SDS‐PAGE. The proposition that fluoride could directly inhibit enzymes was not confirmed in this study
Subject: Esmalte dentário
Peptídeo hidrolases
Fluoretos
Country: Inglaterra
Editor: Wiley
Citation: European Journal Of Oral Sciences. v. 108, n. 1, p. 48-53, 2000-Feb.
Rights: aberto
Identifier DOI: 10.1034/j.1600-0722.2000.00735.x
Address: https://onlinelibrary.wiley.com/doi/abs/10.1034/j.1600-0722.2000.00735.x
Date Issue: 2000
Appears in Collections:FOP - Artigos e Outros Documentos

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