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|Type:||Artigo de periódico|
|Title:||Criteria For Candida Albicans Numerical Analysis Based On Electrophoretic Protein Patterns.|
|Author:||Boriollo, M F|
Rosa, E A
Rosa, R T
Höfling, J F
|Abstract:||Even in normalized gels, some problems with the choice and position of protein bands always have hindered the processing of electrophoretic data. We have developed a way to establish which criterion best fits the necessities in order to maximize the similarity indexes for numerical analysis. Some repetitions of a Candida albicans strain were carried out in eleven different gels. After staining, the bands were scored in numbers within ranges of +/- n values with increases of one quarter steps (+/- 0.25 kDa, +/- 0.5 kDa, +/- 0.75 kDa, +/- 1.0 kDa, +/- 1.25 kDa) that will limit the possibility of variation for the same bands that will appear on the repetitions. Using this criterion, we have determined that values scored within +/- 1.25 kDa could optimize the minimum limiting value of similarity in different dendrograms built.|
Electrophoresis, Polyacrylamide Gel
|Date Issue:||-1-Uns- -1|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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