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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIdentification And Characterization Of The Trns/pseudo-trna/nad3/rps12 Gene Cluster From Coix Lacryma-jobi L: Organization, Transcription And Rna Editing.pt_BR
dc.contributor.authorDias,pt_BR
dc.contributor.authorSiqueira,pt_BR
dc.contributor.authorLejeune,pt_BR
dc.contributor.authorde Souza AP,pt_BR
unicamp.authorDias, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária 'Zeferino Vaz', 13083-970 C.P. 6010 SP, Campinas, Brazilpt_BR
unicamp.author.externalSiqueira,pt
unicamp.author.externalLejeune,pt
unicamp.author.externalde Souza AP,pt
dc.description.abstractDuring a study of mitochondrial sequence conservation between the liverwort Marchantia polymorpha and several Angiosperm species, as revealed by heterologous hybridization experiments, the trnS/pseudo-tRNA/nad3/rps12 gene cluster in Coix lacryma-jobi L., an Asian grass species from the Andropogoneae, was identified using the mitochondrial probe orf167 from M. polymorpha. The Coix gene cluster was cloned and sequenced, and its expression analyzed. The gene sequence and gene locus organization were found to be similar to the corresponding cluster in wheat and maize. Northern hybridization and reverse transcription-polymerase chain reaction analyses indicated that nad3 and rps12 genes were co-transcribed as a 1.25 kb RNA molecule. The transcript displayed 20 and six RNA edition sites, in the nad3 and rps12 genes, respectively, that changed the codon identities to amino acids, which are better conserved in different organisms. Twenty-three cDNA clones were analysed for the edition process and revealed different partial editing patterns without apparent sequential processing.en
dc.relation.ispartofPlant Science : An International Journal Of Experimental Plant Biologypt_BR
dc.relation.ispartofabbreviationPlant Sci.pt_BR
dc.date.issued2000-Seppt_BR
dc.identifier.citationPlant Science : An International Journal Of Experimental Plant Biology. v. 158, n. 1-2, p. 97-105, 2000-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume158pt_BR
dc.description.firstpage97-105pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1873-2259pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/10996249pt_BR
dc.date.available2015-11-27T12:22:40Z-
dc.date.accessioned2015-11-27T12:22:40Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:22:40Z (GMT). No. of bitstreams: 1 pmed_10996249.pdf: 1010645 bytes, checksum: 78e23f593312345b85f5bfa66429939e (MD5) Previous issue date: 2000en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194584-
dc.identifier.idPubmed10996249pt_BR
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