Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194583
Type: Artigo de periódico
Title: Inhibition Of Acid Phosphatase Isoforms Purified From Mature Soybean (glycine Max) Seeds.
Author: Ferreira, C V
Taga, E M
Aoyama, H
Abstract: The four soybean seed acid phosphatase isoforms AP1, AP2, AP3A and AP3B were competitively inhibited by phosphate, vanadate, fluoride and molybdate, using p-nitrophenylphosphate as substrate. The four isoforms were not significantly affected by compounds that can interact with SH residues or by pyridoxal phosphate. These results indicated that cysteine and lysine residues are not present in the active site of the four soybean seed acid phosphatase isoforms. The inhibition constant values for phosphate, vanadate, fluoride and molybdate at pH 5.0 were respectively: API (250, 12.8, 1.7, 0.05 microM). AP2 (800, 10, 500, 0.025 microM), AP3A (250, 24.2,250, 0.032 microM ), AP3B (2400 36.9, 750, 0.05 microM).
Subject: Acid Phosphatase
Enzyme Inhibitors
Fluorides
Isoenzymes
Kinetics
Molybdenum
Nitrophenols
Organophosphorus Compounds
Phosphates
Seeds
Soybeans
Vanadates
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10995070
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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