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Type: Artigo de periódico
Title: New Aqueous Two-phase System Based On Cashew-nut Tree Gum And Poly(ethylene Glycol).
Author: Sarubbo, L A
Oliveira, L A
Porto, A L
Duarte, H S
Carneiro-Leão, A M
Lima-Filho, J L
Campos-Takaki, G M
Tambourgi, E B
Abstract: The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification.
Subject: Carbohydrate Sequence
Hydrogen-ion Concentration
Molecular Sequence Data
Molecular Weight
Polyethylene Glycols
Resins, Plant
Citation: Journal Of Chromatography. B, Biomedical Sciences And Applications. v. 743, n. 1-2, p. 79-84, 2000-Jun.
Rights: fechado
Identifier DOI: 
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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