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Type: Artigo de periódico
Title: Pathways Involved In Trifluoperazine-, Dibucaine- And Praziquantel-induced Hemolysis.
Author: Malheiros, S V
Meirelles, N C
de Paula, E
Abstract: This work elucidates differences in the hemolytic pathway developed by the antipsychotic trifluoperazine (TFP), the local anesthetic dibucaine (DBC) and the antihelminthic praziquantel (PZQ). Their partition coefficients (P) were measured at pH 7.4 between n-octanol, microsomes, liposomes, erythrocyte ghosts and n-octanol/water. The effective drug:lipid molar ratios for the onset of membrane solubilization (ReSAT) and complete hemolysis (ReSOL) were calculated from the experimental P values and compared with a classical surface-active compound treatment Lichtenberg, D. Biochim. Biophys. Acta 821 (1985) 470-478[. The contribution of charged/uncharged forms of TFP and DBC for the hemolytic activity was also analyzed. In all cases the hemolytic phenomena could be related to the monomeric drug insertion into the membrane. Only for TFP at isosmotic condition lysis occurs at concentrations beyond the CMC of the drug, indicating that micellization facilitates TFP hemolytic effect, while DBC and PZQ reach a real membrane saturation at their monomeric form.
Subject: Anesthetics, Local
Antipsychotic Agents
Erythrocyte Membrane
Hydrogen-ion Concentration
Lipid Bilayers
Microsomes, Liver
Citation: Biophysical Chemistry. v. 83, n. 2, p. 89-100, 2000-Jan.
Rights: fechado
Identifier DOI: 
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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