Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194464
Type: Artigo de periódico
Title: Plant Uncoupling Mitochondrial Protein Activity In Mitochondria Isolated From Tomatoes At Different Stages Of Ripening.
Author: Costa, A D
Nantes, I L
Jezek, P
Leite, A
Arruda, P
Vercesi, A E
Abstract: In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and ATP were required to restore deltapsi to the level of that obtained with green tomato mitochondria. This supports the notion that such uncoupling in red tomato mitochondria is mediated by a plant uncoupling mitochondrial protein (PUMP; cf. Vercesi et al., 1995). Nevertheless, mitochondria from both green and red tomatoes exhibited an ATP-sensitive linoleic acid (LA)-induced deltapsi decrease providing evidence that PUMP is also present in green tomatoes. Indeed, proteoliposomes containing reconstituted green or red tomato PUMP showed LA uniport and LA-induced H+ transport. It is suggested that the higher concentration of free fatty acids (PUMP substrates) in red tomatoes could explain the lower coupling state in mitochondria isolated from these fruits.
Subject: Animals
Carrier Proteins
Fatty Acids
Intracellular Membranes
Ion Channels
Lycopersicon Esculentum
Membrane Proteins
Mitochondria
Mitochondrial Proteins
Proteolipids
Protons
Uncoupling Agents
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10653480
Date Issue: 1999
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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