Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194405
Type: Artigo de periódico
Title: Detection Of Cytomegalovirus Infections By Pcr In Renal Transplant Patients.
Author: Costa, S C
Miranda, S R
Alves, G
Rossi, C L
Figueiredo, L T
Costa, F F
Abstract: Cytomegalovirus (CMV) is the single most important infectious agent affecting recipients of organ transplants. To evaluate the incidence and the clinical importance of CMV infection in renal transplants in Brazil, 37 patients submitted to renal allograft transplants were tested periodically for the presence of cytomegalovirus DNA in urine using the polymerase chain reaction (PCR), and for the presence of IgM and IgG antibodies against CMV by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF). The PCR-amplified products were detected by gel electrophoresis and confirmed by dot-blot hybridization with oligonucleotide probes. Thirty-two of the 37 patients (86.4%) were positive by at least one of the three methods. In six patients, PCR was the only test which detected the probable CMV infection. Ten patients had a positive result by PCR before transplantation. In general, the diagnosis was achieved earlier by PCR than by serologic tests. Active infection occurred more frequently during the first four months after transplantation. Sixteen of the 32 patients (50%) with active CMV infection presented clinical symptoms consistent with CMV infection. Five patients without evidence of active CMV infection by the three tests had only minor clinical manifestations during follow-up. Our results indicate that PCR is a highly sensitive procedure for the early detection of CMV infection and that CMV infection in renal transplant patients is a frequent problem in Brazil.
Subject: Cytomegalovirus
Cytomegalovirus Infections
Enzyme-linked Immunosorbent Assay
Humans
Incidence
Kidney Transplantation
Polymerase Chain Reaction
Postoperative Complications
Prevalence
Prospective Studies
Serologic Tests
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10454756
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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