Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194261
Type: Artigo de periódico
Title: Cooperative Dna Binding And Sequence Discrimination By The Opaque2 Bzip Factor.
Author: Yunes, J A
Vettore, A L
da Silva, M J
Leite, A
Arruda, P
Abstract: The maize Opaque2 (O2) protein is a basic leucine zipper transcription factor that controls the expression of distinct classes of endosperm genes through the recognition of different cis-acting elements in their promoters. The O2 target region in the promoter of the alpha-coixin gene was analyzed in detail and shown to comprise two closely adjacent binding sites, named O2u and O2d, which are related in sequence to the GCN4 binding site. Quantitative DNase footprint analysis indicated that O2 binding to alpha-coixin target sites is best described by a cooperative model. Transient expression assays showed that the two adjacent sites act synergistically. This synergy is mediated in part by cooperative DNA binding. In tobacco protoplasts, O2 binding at the O2u site is more important for enhancer activity than is binding at the O2d site, suggesting that the architecture of the O2-DNA complex is important for interaction with the transcriptional machinery.
Subject: Base Sequence
Basic-leucine Zipper Transcription Factors
Binding Sites
Chromosome Mapping
Dna Probes
Dna, Plant
Dna-binding Proteins
Evolution, Molecular
G-box Binding Factors
Gene Expression
Genes, Plant
Leucine Zippers
Molecular Sequence Data
Mutagenesis, Site-directed
Plant Proteins
Promoter Regions, Genetic
Transcription Factors
Zea Mays
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/9811800
Date Issue: 1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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