Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194213
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCharacteristics Of Growth Hormone Binding To Liver Microsomes Of Pregnant Women.pt_BR
dc.contributor.authorD'Abronzo, F Hpt_BR
dc.contributor.authorYamaguchi, M Ipt_BR
dc.contributor.authorAlves, R Spt_BR
dc.contributor.authorSvartman, Rpt_BR
dc.contributor.authorMesquita, C Hpt_BR
dc.contributor.authorNicolau, Wpt_BR
unicamp.authorF H D'Abronzo, Faculdade de Ciências Médicas-UNICAMP, São Paulo, Brazil.pt_BR
unicamp.author.externalM I Yamaguchi,pt
unicamp.author.externalR S Alves,pt
unicamp.author.externalR Svartman,pt
unicamp.author.externalC H Mesquita,pt
unicamp.author.externalW Nicolau,pt
dc.subjectAdultpt_BR
dc.subjectAgedpt_BR
dc.subjectBinding, Competitivept_BR
dc.subjectFemalept_BR
dc.subjectFetuspt_BR
dc.subjectGrowth Hormonept_BR
dc.subjectHumanspt_BR
dc.subjectInfant, Newbornpt_BR
dc.subjectKineticspt_BR
dc.subjectMalept_BR
dc.subjectMicrosomes, Liverpt_BR
dc.subjectMiddle Agedpt_BR
dc.subjectPlacental Lactogenpt_BR
dc.subjectPregnancypt_BR
dc.subjectProlactinpt_BR
dc.subjectReceptors, Somatotropinpt_BR
dc.description.abstractSpecific binding of hGH to liver microsomes of nonpregnant women and men is low, usually 10% of less in RRA. In pregnant women, however, we found that this binding is 10 times higher. The binding reaction shared the properties common to receptor systems: time, temperature and cation dependence, saturability and reversibility, hGH specific binding without cations was 10 times lower. The cross-reactions of hPRL and human placental lactogen with hGH were 0.49 +/- 0.16% and 0.10 +/- 0.05%, respectively. 125I-hPRL and 125I-human placental lactogen binding to microsomes of two controls and two pregnant women were very low and poorly reproducible. The Scatchard analysis revealed two hGH binding sites, one with an association constant (KA) of 2.7 +/- 0.1 x 10(10) M-1, and the other with a KA of 1.5 +/- 0.1 x 10(9) M-1. In one nonpregnant woman, we found a single hGH binding site with a KA of 1.5 x 10(9) M-1, confirming results previously reported in the literature. A hGH RRA was set up with microsomes of pregnant women. Acromegalic sera produced curves parallel to the hGH standard and pituitary dwarf serum had no 125I-hGH displacing activity. Sera of pregnant women produced curves divergent to the hGH standard and showed a 125I-hGH displacing activity 20 to 40 times higher than could be predicted by hGH levels determined by RIA. Cord sera and sera from puerperal women had similar hGH levels as determined by either RRA or RIA (r = 0.93, P less than 0.001, slope = 0.85, n = 25). Our results show the existence of specific GH receptors and serum factor(s) with high 125I-hGH displacing activity from these receptors in pregnancy. These findings must be related to several metabolic changes of pregnancy, such as glucose intolerance, hyperinsulinemia, increased lipolysis, and ketogenesis.en
dc.relation.ispartofThe Journal Of Clinical Endocrinology And Metabolismpt_BR
dc.relation.ispartofabbreviationJ. Clin. Endocrinol. Metab.pt_BR
dc.date.issued1991-Augpt_BR
dc.identifier.citationThe Journal Of Clinical Endocrinology And Metabolism. v. 73, n. 2, p. 348-54, 1991-Aug.pt_BR
dc.language.isoengpt_BR
dc.description.volume73pt_BR
dc.description.firstpage348-54pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0021-972Xpt_BR
dc.identifier.doi10.1210/jcem-73-2-348pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/1856265pt_BR
dc.date.available2015-11-27T12:19:18Z-
dc.date.accessioned2015-11-27T12:19:18Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:19:18Z (GMT). No. of bitstreams: 0 Previous issue date: 1991en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194213-
dc.identifier.idPubmed1856265pt_BR
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