Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194206
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIncreased Tyrosine Phosphorylation Causes Redistribution Of Adherens Junction And Tight Junction Proteins And Perturbs Paracellular Barrier Function In Mdck Epithelia.pt_BR
dc.contributor.authorCollares-Buzato, C Bpt_BR
dc.contributor.authorJepson, M Apt_BR
dc.contributor.authorSimmons, N Lpt_BR
dc.contributor.authorHirst, B Hpt_BR
unicamp.authorC B Collares-Buzato, Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, UK. collares@obelix.unicamp.brpt_BR
unicamp.author.externalM A Jepson,pt
unicamp.author.externalN L Simmons,pt
unicamp.author.externalB H Hirst,pt
dc.subjectActinspt_BR
dc.subjectAnimalspt_BR
dc.subjectCadherinspt_BR
dc.subjectCell Linept_BR
dc.subjectCell Membrane Permeabilitypt_BR
dc.subjectDogspt_BR
dc.subjectGenisteinpt_BR
dc.subjectImmunohistochemistrypt_BR
dc.subjectInulinpt_BR
dc.subjectKidneypt_BR
dc.subjectMembrane Proteinspt_BR
dc.subjectNitrilespt_BR
dc.subjectPeroxidespt_BR
dc.subjectPhalloidinept_BR
dc.subjectPhosphoproteinspt_BR
dc.subjectPhosphorylationpt_BR
dc.subjectPhosphotyrosinept_BR
dc.subjectProtein Tyrosine Phosphatasespt_BR
dc.subjectProtein-tyrosine Kinasespt_BR
dc.subjectRhodaminespt_BR
dc.subjectStaurosporinept_BR
dc.subjectTight Junctionspt_BR
dc.subjectTyrphostinspt_BR
dc.subjectVanadatespt_BR
dc.subjectZonula Occludens-1 Proteinpt_BR
dc.description.abstractPolarized monolayers of strain II Madin-Darby canine kidney cells (MDCK II) were treated with vanadate/H2O2, known inhibitors of protein tyrosine phosphatase activity. Vanadate/H2O2 treatment resulted in a rapid increase in paracellular permeability as revealed by decreased transepithelial resistance and increased permeability to inulin. These alterations in epithelial barrier function coincided with increased phosphotyrosine immunofluorescence in the vicinity of intercellular junctions and with redistribution of F-actin, the adherens junction protein E-cadherin and the tight junction protein ZO-1. The effects of vanadate/H2O2 on intercellular junction permeability and protein distribution were completely blocked by the specific protein tyrosine kinase (PTK) inhibitor tyrphostin 25 and partially inhibited by the alternative PTK inhibitor genistein. The relative potency of these two inhibitors in blocking the effects of vanadate/H2O2 on intercellular junctions correlated with their abilities to inhibit tyrosine phosphorylation. The potent ser/thr protein kinase inhibitor staurosporine had only a small influence on the vanadate/H2O2-induced increase in paracellular permeability and did not affect the observed redistribution of intercellular junction proteins or phosphotyrosine immunofluorescence. The relative potencies of these distinct protein kinase inhibitors in reversing the effects of vanadate/H2O2 indicate that these effects are directly related to tyrosine phosphorylation. In conclusion, our data provide evidence that enhanced tyrosine phosphorylation of intercellular junction proteins in MDCK epithelia increases paracellular permeability and can also induce prominent reorganization of the junctional complex.en
dc.relation.ispartofEuropean Journal Of Cell Biologypt_BR
dc.relation.ispartofabbreviationEur. J. Cell Biol.pt_BR
dc.date.issued1998-Junpt_BR
dc.identifier.citationEuropean Journal Of Cell Biology. v. 76, n. 2, p. 85-92, 1998-Jun.pt_BR
dc.language.isoengpt_BR
dc.description.volume76pt_BR
dc.description.firstpage85-92pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0171-9335pt_BR
dc.identifier.doi10.1016/S0171-9335(98)80020-4pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/9696347pt_BR
dc.date.available2015-11-27T12:19:18Z-
dc.date.accessioned2015-11-27T12:19:18Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:19:18Z (GMT). No. of bitstreams: 1 pmed_9696347.pdf: 2977522 bytes, checksum: 10c905a2e24504ad6714092aaedd714d (MD5) Previous issue date: 1998en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194206-
dc.identifier.idPubmed9696347pt_BR
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