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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePurification From Bothrops Lanceolatus (fer De Lance) Venom Of A Fibrino(geno)lytic Enzyme With Esterolytic Activity.pt_BR
dc.contributor.authorLôbo de Araújo, Apt_BR
dc.contributor.authorDonato, J Lpt_BR
dc.contributor.authorBon, Cpt_BR
unicamp.authorA Lôbo de Araújo, Departamento de Farmacologia, FCM, CP 6111, UNICAMP, Campinas, SP, Brasil.pt_BR
unicamp.author.externalJ L Donato,pt
unicamp.author.externalC Bon,pt
dc.subjectAnimalspt_BR
dc.subjectBothropspt_BR
dc.subjectCrotalid Venomspt_BR
dc.subjectEsterasespt_BR
dc.subjectFibrinolytic Agentspt_BR
dc.subjectHydrolysispt_BR
dc.subjectMolecular Weightpt_BR
dc.subjectRabbitspt_BR
dc.subjectThrombinpt_BR
dc.subjectTosylarginine Methyl Esterpt_BR
dc.description.abstractBothrops lanceolatus venom has high caseinolytic, phospholipasic, esterolytic and hemorrhagic activities. In spite of having no coagulant effect on plasma, this venom contains a thrombin-like enzyme. Using gel filtration and ion-exchange chromatographies, we have purified an esterolytic fraction (F-II-1a) from this venom with a protein yield of 4% and a 58% recovery in enzyme activity. SDS-PAGE in the presence of beta-mercaptoethanol showed that the enzyme is a single chain polypeptide with a MW=38,100. Immunodiffusion and immunoelectrophoresis of fraction F-II-1a against serum from horses immunized with B. lanceolatus venom and against rabbit antiserum prepared using fraction F-II-1a both showed a single immunoprecipitin line. The Km and Vmax values for TAME hydrolysis were 0.85 mM and 38.6 micromol/min/mg, respectively. The esterolytic activity was completely inhibited by PMSF (10 mM) but not by EDTA (20 mM). Fraction F-II-1a hydrolyzed the alpha and beta chains of fibrinogen. Degradation of the alpha chain occurred within 10 min while that of the beta-chain was slower. The enzyme had no effect on the gamma-chain even after 4 h of hydrolysis.en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.date.issued1998-Maypt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 36, n. 5, p. 745-58, 1998-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume36pt_BR
dc.description.firstpage745-58pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/9655635pt_BR
dc.date.available2015-11-27T12:19:15Z-
dc.date.accessioned2015-11-27T12:19:15Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:19:15Z (GMT). No. of bitstreams: 1 pmed_9655635.pdf: 462765 bytes, checksum: e208842bd2793a4e29ee746391d2bf95 (MD5) Previous issue date: 1998en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194184-
dc.identifier.idPubmed9655635pt_BR
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