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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleInhibition Of Succinic Dehydrogenase And F0f1-atp Synthase By 4,4'-diisothiocyanatostilbene-2,2'-disulfonic Acid (dids).pt_BR
dc.contributor.authorBernardes, C Fpt_BR
dc.contributor.authorMeyer-Fernandes, J Rpt_BR
dc.contributor.authorMartins, O Bpt_BR
dc.contributor.authorVercesi, A Ept_BR
unicamp.authorC F Bernardes, Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Brazil.pt_BR
unicamp.author.externalJ R Meyer-Fernandes,pt
unicamp.author.externalO B Martins,pt
unicamp.author.externalA E Vercesi,pt
dc.subject4,4'-diisothiocyanostilbene-2,2'-disulfonic Acidpt_BR
dc.subjectAnimalspt_BR
dc.subjectCattlept_BR
dc.subjectEnzyme Inhibitorspt_BR
dc.subjectKineticspt_BR
dc.subjectMitochondria, Heartpt_BR
dc.subjectMitochondria, Liverpt_BR
dc.subjectOxygen Consumptionpt_BR
dc.subjectProton-translocating Atpasespt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectSubmitochondrial Particlespt_BR
dc.subjectSuccinate Dehydrogenasept_BR
dc.description.abstractThis study shows that incubation of rat liver mitochondria in the presence of the thiol/ amino reagent 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) is followed by inhibition of both succinate supported respiration and oxidative phosphorylation. Half-maximal inhibition of succinic dehydrogenase activity and succinate oxidation by mitochondria was attained at 55.3 and 60.8 microM DIDS, respectively. DIDS did inhibit the net ATP synthesis and ATP<=>[32P]Pi exchange reaction catalyzed by submitochondrial particles in a dose-dependent manner (Ki = 31.7 microM and Ki = 32.7 microM), respectively. The hydrolytic activities of uncoupled heart submitochondrial particles and purified F1-ATPase were also inhibited 50% by 31.9 and 20.9 microM DIDS, respectively.en
dc.relation.ispartofZeitschrift Für Naturforschung. C, Journal Of Biosciencespt_BR
dc.relation.ispartofabbreviationZ. Naturforsch., C, J. Biosci.pt_BR
dc.date.issuedpt_BR
dc.identifier.citationZeitschrift Für Naturforschung. C, Journal Of Biosciences. v. 52, n. 11-12, p. 799-806pt_BR
dc.language.isoengpt_BR
dc.description.volume52pt_BR
dc.description.firstpage799-806pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0939-5075pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/9463937pt_BR
dc.date.available2015-11-27T12:19:04Z-
dc.date.accessioned2015-11-27T12:19:04Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:19:04Z (GMT). No. of bitstreams: 0 Previous issue date: nullen
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194087-
dc.identifier.idPubmed9463937pt_BR
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