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Type: Artigo de periódico
Title: Inhibition Of Succinic Dehydrogenase And F0f1-atp Synthase By 4,4'-diisothiocyanatostilbene-2,2'-disulfonic Acid (dids).
Author: Bernardes, C F
Meyer-Fernandes, J R
Martins, O B
Vercesi, A E
Abstract: This study shows that incubation of rat liver mitochondria in the presence of the thiol/ amino reagent 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) is followed by inhibition of both succinate supported respiration and oxidative phosphorylation. Half-maximal inhibition of succinic dehydrogenase activity and succinate oxidation by mitochondria was attained at 55.3 and 60.8 microM DIDS, respectively. DIDS did inhibit the net ATP synthesis and ATP<=>[32P]Pi exchange reaction catalyzed by submitochondrial particles in a dose-dependent manner (Ki = 31.7 microM and Ki = 32.7 microM), respectively. The hydrolytic activities of uncoupled heart submitochondrial particles and purified F1-ATPase were also inhibited 50% by 31.9 and 20.9 microM DIDS, respectively.
Subject: 4,4'-diisothiocyanostilbene-2,2'-disulfonic Acid
Enzyme Inhibitors
Mitochondria, Heart
Mitochondria, Liver
Oxygen Consumption
Proton-translocating Atpases
Rats, Wistar
Submitochondrial Particles
Succinate Dehydrogenase
Citation: Zeitschrift Für Naturforschung. C, Journal Of Biosciences. v. 52, n. 11-12, p. 799-806
Rights: fechado
Identifier DOI: 
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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