Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194065
Type: Artigo de periódico
Title: Large-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain.
Author: Miranda, E A
de-Murcia, G
Ménissier-de-Murcia, J
Abstract: A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 10(9) infected cells in three liters of culture.
Subject: Animals
Baculoviridae
In Vitro Techniques
Insects
Poly Adenosine Diphosphate Ribose
Polynucleotide Adenylyltransferase
Recombinant Proteins
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/9361719
Date Issue: 1997
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_9361719.pdf221.64 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.