Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193969
Type: Artigo de periódico
Title: Adhesion Of Bovine Enterotoxigenic Escherichia Coli (etec) By Type 1-like Fimbriae.
Author: Catani, C F
Yamada, A T
Vidotto, M C
Yano, T
Abstract: Enterotoxigenic Escherichia coli (STa+) strains were isolated from adult bovine with diarrhea. These strains did not express any known ETEC-specific adhesins. Although hemagglutination with rat and sheep erythrocytes was observed in the presence of D-mannose (MRHA), these strains also showed mannose-sensitive hemagglutination (MSHA) with guinea-pig erythrocytes. Electron microscopic studies revealed the presence of fimbria-like structures (provisionally called F43ms) on bacterial cells grown at 37 degrees C but not on cells grown at 18 degrees C. However, it was observed by SDS-PAGE that the J-1 strain (F43ms+) produces a protein similar to F1 fimbriae, and this strain hybridized with a DNA probe for F1 fimbriae. Immunogold-labelling techniques indicated that a rabbit anti-serum is specific for F43ms fimbrial structures, but not for Type 1 fimbriae. The immunofluorescence test carried out with semipurified F43ms on bovine brush borders suggests that the fimbria-like structures are responsible for the adhesion to bovine epithelial cells.
Subject: Animals
Bacterial Adhesion
Cattle
Cattle Diseases
Escherichia Coli
Escherichia Coli Infections
Fimbriae, Bacterial
Guinea Pigs
Hemagglutination
Humans
In Vitro Techniques
Mannose
Microscopy, Electron
Rabbits
Rats
Sheep
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/8998992
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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