Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193965
Type: Artigo de periódico
Title: Rapid Detection Of Factor V Leiden (fvq506) By Non-radioactive Single Strand Conformation Polymorphism (sscp).
Author: Arruda, V R
von Zuben, P M
Annichino-Bizzachi, J M
Costa, F F
Abstract: The transition G-->A at position 506 of the factor V gene is responsible for resistance to the anticoagulant effect of activated protein C (APC-resistance), and represents the most common hereditary risk factor for venous thrombosis. A comparison of the ability of non-radioactive single-strand conformation polymorphism (SSCP) and of a standard PCR procedure followed by further digestion with MnlI to detect this factor V gene (FVQ 506) transition indicates that these is a good agreement between the two methods. Non-radioactive SSCP analysis therefore represents a rapid and sensitive alternative for the diagnosis of this important point mutation.
Subject: Dna Mutational Analysis
Deoxyribonucleases, Type Ii Site-specific
Disease Susceptibility
Factor V
Factor V Deficiency
Humans
Point Mutation
Polymorphism, Single-stranded Conformational
Silver Staining
Thromboembolism
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/8986114
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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