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Type: Artigo de periódico
Title: Evaluation Of Recent Techniques For Detection Of Red Blood Cell Antibodies In Sera Of Reference Samples, Patients, Pregnant Women, And Blood Donors.
Author: de Castilho, L M
Pellegrino, J
Bechelli, A P
Le Pennec, P Y
Mendes, N F
Abstract: The sensitivity of the low ionic strength solution antiglobulin test (LISS-AGT), polyethylene glycol antiglobulin test (PEG-AGT), low ionic strength solution solid-phase antiglobulin test (LISS-SPAT), gel low ionic strength solution antiglobulin test (GEL-LISS), and gel papain test (GEL-PAP) was compared in titration studies of 460 sera containing identified IgG alloantibodies. The GEL-PAP was 100% sensitive to detect Rh antibodies, whereas the PEG-AGT was the most sensitive to detect Kell, Duffy, Kidd, Ss, and rare blood group antibodies. The better performance of PEG-AGT was especially obvious with Kell, Duffy, and Ss antibodies (S = 100%). When the sensitivity of the LISS-AGT, PEG-AGT, GEL-LISS, and GEL-PAP was evaluated in different routines, the GEL-LISS showed to be more sensitive than PEG-AGT in the detection of clinically significant antibodies. These discrepant results showed that the performance of a technique may change when it is applied as a routine.
Subject: Blood Donors
Sensitivity And Specificity
Rights: fechado
Identifier DOI: 10.1002/(SICI)1098-2825(1996)10:5<250::AID-JCLA4>3.0.CO;2-C
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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