Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193906
Type: Artigo de periódico
Title: Insulin Induces Tyrosine Phosphorylation Of Jak2 In Insulin-sensitive Tissues Of The Intact Rat.
Author: Saad, M J
Carvalho, C R
Thirone, A C
Velloso, L A
Abstract: The Janus kinase family of protein tyrosine kinases constitutes a novel type of signal transduction pathway activated in response to a wide variety of polypeptide ligands and has four known members: JAK1, JAK2, JAK3, and Tyk2. In this study, we examined the ability of insulin to stimulate JAK2 tyrosine phosphorylation in insulin-sensitive tissues of the intact rat using immunoprecipitation and immunoblotting. The results demonstrate that after an infusion of insulin, JAK2 is rapidly tyrosine phosphorylated (and the kinase is activated) in the liver, adipose tissue, skeletal muscle, heart, and isolated adipocytes. The presence of phosphorylated JAK2 was detectable after an infusion of insulin that increased serum insulin to physiological postprandial levels (40-70 microunits/ml). Co-immunoprecipitation with anti-insulin receptor antibody, anti-JAK2 antibody, and anti-IRS-1 antibody showed that JAK2 interacts with the insulin receptor and IRS-1 to form stable complexes following stimulation by insulin. In two animal models of insulin resistance the regulation of JAK2 tyrosine phosphorylation after insulin infusion paralleled the phosphorylation of the insulin receptor and of IRS-1. In conclusion, our data indicate that after physiological stimulation by insulin in the intact animal, JAK2 associates with the insulin receptor and is tyrosine phosphorylated in insulin-sensitive tissues in a time- and dose-dependent fashion.
Subject: Adipose Tissue
Animals
Dose-response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Insulin
Janus Kinase 2
Liver
Male
Muscle, Skeletal
Myocardium
Phosphorylation
Protein-tyrosine Kinases
Proto-oncogene Proteins
Rats
Signal Transduction
Time Factors
Tyrosine
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/8703019
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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