Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193898
Type: Artigo de periódico
Title: Opening Of The Mitochondrial Permeability Transition Pore By Uncoupling Or Inorganic Phosphate In The Presence Of Ca2+ Is Dependent On Mitochondrial-generated Reactive Oxygen Species.
Author: Kowaltowski, A J
Castilho, R F
Vercesi, A E
Abstract: In this study, we show that mitochondrial membrane permeability transition in Ca(2+)-loaded mitochondria treated with carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or inorganic phosphate (P(i)) is preceded by enhanced production of H2O2. This production is inhibited either by ethylene glycobis(b-aminoethyl ether)N,N,N',N'-tetraacetic acid (EGTA) or Mg2+, but not by cyclosporin A. Permeability transition is prevented either by EGTA, catalase or dithiothreitol, suggesting the involvement of Ca2+, H2O2 and oxidation of membrane protein thiols in this mechanism. When mitochondria are incubated under anaerobiosis, no permeabilization or H2O2 production occurs. Based on these results we conclude that mitochondrial permeability transition induced by P(i) or FCCP-uncoupling is dependent on mitochondrial-generated reactive oxygen species.
Subject: Animals
Calcium
Carbonyl Cyanide P-trifluoromethoxyphenylhydrazone
Catalase
Dithiothreitol
Egtazic Acid
Hydrogen Peroxide
Intracellular Membranes
Magnesium
Mitochondria, Liver
Permeability
Phosphates
Rats
Rats, Wistar
Reactive Oxygen Species
Uncoupling Agents
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/8549822
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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