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Type: Artigo de periódico
Title: A Quick Procedure For The Isolation Of Dimeric Piratoxins-i And Ii, Two Myotoxins From Bothrops Pirajai Snake Venom. N-terminal Sequencing.
Author: Toyama, M H
Mancuso, L C
Giglio, J R
Novello, J C
Oliveira, B
Marangoni, S
Abstract: Two myotoxins, MP-I and MP-II, from Bothrops pirajai snake venom, have been purified by a quick high performance liquid chromatography (HPLC) procedure. Based on the HPLC coelution profile, amino acid composition, N-terminal sequence, polyacrylamide gel electrophoresis (PAGE) migration, as well as lack of phospholipase-A2 (PLA2) and proteolytic activities, MP-I and MP-II were identified as piratoxin-I (PrTX-I) and II (PrTX-II), respectively. This procedure affords, aside the reduced operation time, a high yield (35% of the applied sample in terms of A280nm) of MP-I, which is the major myotoxin of the venom. The N-terminal sequences of MP-I, MP-II, PrTX-I and PrTX-II, up to the 51st, 41st, 46th and 39th residues, respectively, have been determined, revealing MP-I (and hence PrTX-I) as a Lys-49 PLA2-like myotoxin. Both MP-I and MP-II have been shown, by SDS-PAGE, to occur in dimeric isoforms.
Subject: Amino Acid Sequence
Amino Acids
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Group Ii Phospholipases A2
Molecular Sequence Data
Phospholipases A
Phospholipases A2
Reptilian Proteins
Time Factors
Citation: Biochemistry And Molecular Biology International. v. 37, n. 6, p. 1047-55, 1995-Dec.
Rights: fechado
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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