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Type: Artigo de periódico
Title: Optical Anisotropy Of Toluidine Blue-stained Transformed Cell Nuclei.
Author: Mello, M L
Abstract: Changes in chromatin order with cell transformation were studied in terms of optical anisotropy (birefringence) in toluidine blue-stained NIH 3T3 and Balb/3T3 cells. The transformed NIH 3T3 cell lines used were obtained by transfection with the T24 cell H-ras oncogene and the whole genomic DNA of MCF-7 human breast carcinoma cells. C2PO and PAP2 cell lines were used as representatives of Ha-ras-transformed NIH 3T3 cells with poor and high metastatic ability, respectively. The chromatin of all cells examined exhibited metachromasy and a birefringence of greenish-yellow interference color. The highest birefringence intensity was found in the chromatin granules of the most frequent nuclear phenotype of ras- and MCF-7 DNA-transformed cells, with the exception of the cells with high metastatic ability, in which a faint birefringence was observed. The differences in chromatin birefringence intensity are assumed to indicate differences in chromatin stereoarrangement with cell transformation. In the case of the highly tumorigenic and/or highly metastatic transformants the faint birefringence is assumed to be associated with the heterogeneous and complex physiological processes that may require a relatively less ordered arrangement of the chromatin.
Subject: 3t3 Cells
Breast Neoplasms
Cell Nucleus
Cell Transformation, Neoplastic
Dna, Neoplasm
Genes, Ras
Mice, Inbred Balb C
Mice, Inbred Strains
Tolonium Chloride
Tumor Cells, Cultured
Rights: fechado
Identifier DOI: 10.1016/S0065-1281(11)80093-0
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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