Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193821
Type: Artigo de periódico
Title: Isolation Of A Polypeptide From Phoneutria Nigriventer Spider Venom Responsible For The Increased Vascular Permeability In Rabbit Skin.
Author: Bento, A C
Rego, E
Pedroso-Mariani, S R
Mancuso, L C
Giglio, J R
Novello, J C
Marangoni, S
Caracelli, I
Oliveira, B
Antunes, E
Abstract: Fractionation of Phoneutria nigriventer venom by Sephadex G-10 followed by ion-exchange chromatography yields a fraction (fraction XIII) which increases microvascular permeability in rabbit skin in vivo by activating the tissue kallikrein-kinin system. One polypeptide (PNV3) with the ability to increase microvascular permeability in the rabbit skin in vivo was isolated from fraction XIII and biochemically characterized. PNV3 has 132 amino acid residues with a calculated mol. wt of 14,475. This polypeptide showed the following N-terminal sequence: AVFAIQDQPC. Amino acid analysis indicated the presence of six disulfide bridges and a high content of Glx (20%). Pairwise comparison of PNV3 amino acid sequence with 27 other spider venom polypeptides and proteins indicated that PNV3 presents high similarity (60-70%) with other toxins (Tx2.1, Tx2.5 and Tx2.6) isolated from P. nigriventer venom.
Subject: Amino Acid Sequence
Amino Acids
Animals
Capillary Permeability
Chromatography, Gel
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Molecular Weight
Peptide Hydrolases
Peptides
Rabbits
Skin
Spider Venoms
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/7597720
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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