Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193814
Type: Artigo de periódico
Title: Re-evaluating The Agnor Staining Response In Triton X-100-treated Liver Cells By Image Analysis.
Author: Vidal, B de C
Mello, M L
Abstract: The response of liver cell nuclei to a variant of the AgNOR method, which includes a treatment with Triton X-100 prior to staining assumed to improve the quality of the AgNOR-positive images, was compared to that of the usual method by image analysis procedures. The objective was to determine whether Triton X-100 removes proteins from the nuclei to the point of affecting the AgNOR response. Statistically significant differences were not found in comparisons of a nuclear area/AgNOR-stained area ratio nor in the number of AgNOR-positive dots/aggregates. It was thus assumed that the Triton X-100 treatment did not remove nuclear proteins likely to affect the AgNOR response, though it induced a cleaner background and, consequently, a clearer contrast between the stained areas and the background.
Subject: Animals
Image Processing, Computer-assisted
Liver
Mice
Nucleolus Organizer Region
Octoxynol
Silver Staining
Citation: Analytical Cellular Pathology : The Journal Of The European Society For Analytical Cellular Pathology. v. 9, n. 1, p. 39-43, 1995-Jul.
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/7577753
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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