Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193789
Type: Artigo de periódico
Title: Targeted Development Of Microsatellite Markers From Inter-alu Amplification Of Yac Clones.
Author: de Souza, A P
Allamand, V
Richard, I
Brenguier, L
Chumakov, I
Cohen, D
Beckmann, J S
Abstract: Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
Subject: Base Sequence
Chromosome Mapping
Chromosomes, Artificial, Yeast
Cloning, Molecular
Dna, Satellite
Gene Amplification
Genetic Markers
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Rights: fechado
Identifier DOI: 10.1006/geno.1994.1079
Address: http://www.ncbi.nlm.nih.gov/pubmed/8188274
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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