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Type: Artigo de periódico
Title: Crotoxin Induces Aggregation Of Human Washed Platelets.
Author: Landucci, E C
Condino-Neto, A
Perez, A C
Hyslop, S
Corrado, A P
Novello, J C
Marangoni, S
Oliveira, B
Antunes, E
de Nucci, G
Abstract: Crotoxin, the main toxic component isolated from the venom of the South American rattlesnake Crotalus durissus terrificus, is a reversible protein complex composed of a non-toxic non-enzymatic acidic polypeptide (crotapotin) and a toxic basic phospholipase A2 (PLA2). In this study, we have evaluated the ability of crotoxin to induced aggregation in human washed platelets. Human washed platelet aggregation was monitored in a Payton aggregometer and thromboxane B2 (TXB2) release measured by direct radioimmunoassay (RIA). Crotoxin (15-50 micrograms/ml) produced dose-dependent and irreversible human washed platelet aggregation, which was inhibited by pre-incubation of the platelets with sodium nitroprusside (50-500 microM) or iloprost (8-80 nM). Crotoxin also induced TXB2 release (207 +/- 8 ng/ml, n = 6), and although indomethacin significantly reduced the release of TXB2 (to 23.5 +/- 5 ng/ml, P < 0.001, n = 6), it did not inhibit crotoxin-induced aggregation. Our results clearly demonstrate that crotoxin induces human washed platelet aggregation and that this phenomenon is independent of the formation of pro-aggregatory arachidonic acid metabolites.
Subject: Amino Acid Sequence
Chromatography, High Pressure Liquid
In Vitro Techniques
Molecular Sequence Data
Phospholipases A
Phospholipases A2
Platelet Aggregation
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 32, n. 2, p. 217-26, 1994-Feb.
Rights: fechado
Identifier DOI: 
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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