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Type: Artigo de periódico
Title: The Transcriptional Activator Opaque2 Recognizes Two Different Target Sequences In The 22-kd-like Alpha-prolamin Genes.
Author: Yunes, J A
Cord Neto, G
da Silva, M J
Leite, A
Ottoboni, L M
Arruda, P
Abstract: The maize Opaque2 (O2) protein is a leucine zipper DNA binding factor that interacts with the sequence TCCACGTAGA in the promoters of the 22-kD alpha-zein genes and activates its transcription. A completely different consensus sequence (GATGAPyPuTGPu) identified in b-32, a gene that encodes an abundant albumin that is also under control of the O2 locus, can also be bound by the O2 protein. We showed that the gene encoding the 22-kD-like alpha-coixin, the alpha-prolamin of the maize-related grass Coix, can also be transactivated by the O2 protein. A binding assay in vitro and footprint analysis demonstrated that the GACATGTC sequence of the alpha-coixin promoter can be recognized and protected by the maize O2 protein. Employing transient expression experiments in immature maize endosperm and tobacco mesophyll protoplasts, we demonstrated that the O2 protein can activate expression of the beta-glucuronidase reporter gene placed under the control of the 22-kD-like alpha-coixin promoter. We also demonstrated that a 22-kD-like alpha-coixin pseudogene promoter is transactivated by the maize O2 protein.
Subject: Base Sequence
Dna-binding Proteins
Gene Expression Regulation
Leucine Zippers
Molecular Sequence Data
Plant Proteins
Promoter Regions, Genetic
Restriction Mapping
Sequence Homology, Nucleic Acid
Transcription Factors
Transcription, Genetic
Zea Mays
Rights: fechado
Identifier DOI: 
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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