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Type: Artigo de periódico
Title: The Kinetics Of Chondroitin 4-sulfate Release From Stimulated Platelets And Its Relation To Thromboxane A2 Formation And Granule Secretion.
Author: Donato, J L
Nogueira, M D
Marcondes, S
Antunes, E
Nader, H B
Dietrich, C P
de Nucci, G
Abstract: 1. In platelet rich plasma (PRP), chondroitin 4-sulfate release from platelets occurred after stimulation with ADP (5 microM), collagen (5-10 micrograms/ml), or adrenaline (10 microM). Release started within 60 s and maximum release (0.7-2.0 mg/l) was reached within 180 s. TXA2 formation and dense granule release reached a maximum within 90 s after stimulation. 2. Using washed platelets (1.5 x 10(8) cells/ml), the platelet responses were faster. Release of chondroitin 4-sulfate and TXA2 started within 20-30 s after thrombin addition (100 mU/ml). Maximum release was reached within 60 s in both cases. Dense granule release started in the first 5 s of stimulation (34.6 +/- 12.4%) reaching maximum secretion (74.4 +/- 8.7%) within 60 s. 3. Our results demonstrate that maximal chondroitin 4-sulfate release occurs after the dense granule release reaction in both PRP and washed platelets. This observation suggests that chondroitin 4-sulfate is unlikely to be stored in the dense granules but may be stored in the alpha-granules.
Subject: Adenosine Diphosphate
Blood Platelets
Chondroitin Sulfates
Cytoplasmic Granules
Platelet Aggregation
Thromboxane A2
Time Factors
Citation: Brazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 27, n. 9, p. 2163-7, 1994-Sep.
Rights: aberto
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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