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Type: Artigo de periódico
Title: Immunological Response To Cell-free Antigens Of Paracoccidioides Brasiliensis: Relationship With Clinical Forms Of Paracoccidioidomycosis.
Author: Blotta, M H
Camargo, Z P
Abstract: Sera from patients with the acute (AF) and chronic (CF) forms of paracoccidioidomycosis (PCM) were tested against Paracoccidioides brasiliensis cell-free antigens by Western blot (immunoblot). The CFA preparation contained components ranging in molecular mass from 18 to 102 kDa. The immunoglobulin G (IgG) reactivity profiles were similar for patients with both forms of the disease, and the 43-kDa component was recognized by 100% of the sera. IgM antibodies from the AF- and the CF-PCM sera recognized 21 and 20 components, respectively, the AF-PCM sera reacting preferentially with components with molecular masses above 50 kDa. None of the AF-PCM sera (IgM) reacted with the 43-kDa component, and only 10% of the CF-PCM sera recognized this molecule. The IgA response was more significant in the CF-PCM group than in the AF-PCM group, and the 43- and 74-kDa components were the most reactive ones (about 40% each). Our results showed that the cell-free antigen preparation is very appropriate for the immunoblotting analysis of PCM sera, and they also showed that the detection of IgG anti-gp43 is the best marker for the diagnosis and the following up of patients with the acute or the chronic form of the disease.
Subject: Acute Disease
Antibodies, Fungal
Antigens, Fungal
Blotting, Western
Chronic Disease
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Middle Aged
Citation: Journal Of Clinical Microbiology. v. 31, n. 3, p. 671-6, 1993-Mar.
Rights: aberto
Identifier DOI: 
Date Issue: 1993
Appears in Collections:Unicamp - Artigos e Outros Documentos

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