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Type: Artigo de periódico
Title: The Plant Mitochondrial Open Reading Frame Orf221 Encodes A Membrane-bound Protein.
Author: Prioli, L M
Huang, J
Levings, C S
Abstract: We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.
Subject: Cell Fractionation
Dna, Mitochondrial
Escherichia Coli
Genes, Plant
Membrane Proteins
Open Reading Frames
Protein Biosynthesis
Recombinant Fusion Proteins
Zea Mays
Rights: fechado
Identifier DOI: 
Date Issue: 1993
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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