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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleLipoxygenase-derived Mediators May Be Involved In In Vivo Neutrophil Migration Induced By Bothrops Erythromelas And Bothrops Alternatus Venoms.pt_BR
dc.contributor.authorFlores, C Apt_BR
dc.contributor.authorZappellini, Apt_BR
dc.contributor.authorPrado-Franceschi, Jpt_BR
unicamp.authorC A Flores, Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas-SP, Brazil.pt_BR
unicamp.author.externalA Zappellini,pt
unicamp.author.externalJ Prado-Franceschi,pt
dc.subjectAnimalspt_BR
dc.subjectArachidonic Acidspt_BR
dc.subjectBothropspt_BR
dc.subjectChemotactic Factorspt_BR
dc.subjectChemotaxis, Leukocytept_BR
dc.subjectCrotalid Venomspt_BR
dc.subjectDexamethasonept_BR
dc.subjectFemalept_BR
dc.subjectIndomethacinpt_BR
dc.subjectLipoxygenasept_BR
dc.subjectMacrophages, Peritonealpt_BR
dc.subjectMalept_BR
dc.subjectMasoprocolpt_BR
dc.subjectNeutrophilspt_BR
dc.subjectPhospholipases Apt_BR
dc.subjectPhospholipases A2pt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectThioglycolatespt_BR
dc.description.abstractBothrops erythromelas (BEV) and B. alternatus (BAV) venoms induced a dose-dependent neutrophil migration when injected into rat peritoneal cavities (20-160 micrograms/cavity). These venoms (80 micrograms/rat) also induced neutrophil migration in the air pouch model of inflammation. This migratory response seemed to be related to the phospholipase A2 (PLA2) activity of the venoms. BAV had approximately two times more PLA2 activity than BEV, and the neutrophil migration induced by the former venom was two to three-fold greater than that observed with the latter. Heated (90 degrees C for 5 min) BEV lost about 50% of its PLA2 activity and this was accompanied by a corresponding loss in the ability to induce neutrophil chemotaxis. Dexamethasone (0.5 mg/kg, s.c.), an indirect inhibitor of PLA2 activity, also abolished the neutrophil migration induced by both venoms. Since NDGA (100 mg/kg, s.c.) and dexamethasone, but not indomethacin (2 mg/kg, s.c.), strongly reduced the neutrophil migration induced by both bothropic venoms, it is suggested that arachidonate-derived lipoxygenase metabolites such as leukotriene B4 act as the chemotactic mediators. Macrophages could be the main cellular source of such metabolites since they are the predominant resident cells in the rat air pouch, and the migratory response of BEV and BAV into peritoneal cavities was potentiated in rats pretreated with thioglycollate. The neutrophil migration induced by BEV and BAV was not due to endotoxin contamination since heated BEV showed no effect and polymyxin B-treated BAV still remained active.en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.date.issued1993-Decpt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 31, n. 12, p. 1551-9, 1993-Dec.pt_BR
dc.language.isoengpt_BR
dc.description.volume31pt_BR
dc.description.firstpage1551-9pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/8146868pt_BR
dc.date.available2015-11-27T12:18:18Z-
dc.date.accessioned2015-11-27T12:18:18Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:18:18Z (GMT). No. of bitstreams: 0 Previous issue date: 1993en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193691-
dc.identifier.idPubmed8146868pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

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