Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193670
Type: Artigo de periódico
Title: Cloning And Characterization Of A Cdna Encoding A Sulfur-rich Coixin.
Author: Leite, A
Yunes, J A
Turcinelli, S R
Arruda, P
Abstract: A full-length cDNA clone encoding a sulfur-rich Coix prolamin was isolated using a cDNA library constructed from polysomal mRNA prepared from immature Coix endosperm. The deduced amino acid sequence of the cDNA clone predicted a polypeptide of 194 residues, which shared 64% homology with the 17 kDa beta-zein. The mature protein contains the familiar composition of the prolamins and an unusually high content of the sulfur-containing amino acids methionine (11.6%) and cysteine (5.2%). In vitro transcription followed by in vitro translation of the coding region of the pBCX17.9S clone gave rise to a polypeptide with an apparent molecular weight corresponding to the C4 alpha-coixin. Hydropathy analysis showed that C4 alpha-coixin is slightly more hydrophobic than beta-zein.
Subject: Amino Acid Sequence
Base Sequence
Cloning, Molecular
Molecular Sequence Data
Plant Proteins
Poaceae
Sequence Homology, Nucleic Acid
Zein
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/1731973
Date Issue: 1992
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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