Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193658
Type: Artigo de periódico
Title: Calcium-dependent Mitochondrial Oxidative Damage Promoted By 5-aminolevulinic Acid.
Author: Hermes-Lima, M
Castilho, R F
Valle, V G
Bechara, E J
Vercesi, A E
Abstract: Swelling of isolated rat liver mitochondria is shown to be induced by metal-catalyzed 5-aminolevulinic acid (ALA) aerobic oxidation, a putative endogenous source of reactive oxygen species (ROS), at concentrations as low as 50-100 microM. In this concentration range, ALA is estimated to occur in the liver of acute intermittent porphyria patients. Removal of Ca2+ (10 microM) from the suspension of isolated rat liver mitochondria by added EGTA abolishes both the ALA-induced transmembrane-potential collapse and mitochondrial swelling. Prevention of the ALA-induced swelling by addition of ruthenium red prior to mitochondrial energization by succinate demonstrates the deleterious involvement of internal Ca2+. Addition of MgCl2 at concentrations higher than 2.5 mM, prevents the ALA-induced mitochondrial swelling, transmembrane potential collapse and Ca2+ efflux. This indicates that Mg2+ protects against the mitochondrial damage promoted by ALA-generated ROS. The ALA-induced mitochondrial damage might be a key event in the liver mitochondrial damage of acute intermittent porphyria patients reported elsewhere.
Subject: Aminolevulinic Acid
Animals
Calcium
Cell Membrane Permeability
Cell Size
Egtazic Acid
Magnesium
Male
Mitochondria, Liver
Oxidation-reduction
Porphyrias, Hepatic
Rats
Rats, Wistar
Reactive Oxygen Species
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/1463771
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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