Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Proteomic analysis of papaya fruit ripening using 2DE-DIGE
Author: Nogueira, Silvia Beserra
Labate, Carlos Alberto
Gozzo, Fabio Cesar
Pilau, Eduardo Jorge
Lajolo, Franco Maria
Oliveira do Nascimento, Joao Roberto
Abstract: Papayas have a very short green life as a result of their rapid pulp softening as well as their susceptibility to physical injury and mold growth. The ripening-related changes take place very quickly, and there is a continued interest in the reduction of postharvest losses. Proteins have a central role in biological processes, and differential proteomics enables the discrimination of proteins affected during papaya ripening. A comparative analysis of the proteomes of climacteric and pre-climacteric papayas was performed using 2DE-DIGE. Third seven proteins corresponding to spots with significant differences in abundance during ripening were submitted to MS analysis, and 27 proteins were identified and classified into six main categories related to the metabolic changes occurring during ripening. Proteins from the cell wall (alpha-galactosidase and invertase), ethylene biosynthesis (methionine synthase), climacteric respiratory burst, stress response, synthesis of carotenoid precursors (hydroxymethylbutenyl 4-diphosphate synthase, GcpE), and chromoplast differentiation (fibrillin) were identified. There was some correspondence between the identified proteins and the data from previous transcript profiling of papaya fruit, but new, accumulated proteins were identified, which reinforces the importance of differential proteomics as a tool to investigate ripening and provides potentially useful information for maintaining fruit quality and minimizing postharvest losses. (C) 2011 Elsevier B.V. All rights reserved.
Subject: Papaya ripening
Papaya proteome
MEP pathway
Editor: Elsevier
Citation: Journal of Proteomics. Elsevier, v.75, n.4, p.1428-1439, 2012
Rights: fechado
Identifier DOI: 10.1016/j.jprot.2011.11.015
Date Issue: 2012
Appears in Collections:IQ - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.