Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/1839
Type: Artigo de periódico
Title: Experimental model of smoking and simulation of reflux with acid and pepsin in rats
Author: Zen Junior, Jose Helio
Del Negro, Andre
Colli Neto, Jose Alexandre
Araujo, Marina Rachel
Altemani, Albina Maria
Andreollo, Nelson Adami
Abstract: PURPOSE: To develop experimental models to evaluate the effects of hydrochloric acid associated with the pepsin instilled in the mucosa of the upper esophagus and the esophagogastric junction of young male rats Wistar, simulating injury caused by gastroesophageal reflux on the mucosa of aero-digestive tract in humans as well as the action of the risk exposure of mucosa to cigarette smoke. METHODS: Fifty young male Wistar rats divided in 5 groups with 10 animals each one, respectively simulating pharyngo-laryngeal reflux and gastroesophageal reflux, pharyngo-laryngeal reflux and smoking, smoking only, gastroesophageal reflux and control group. RESULTS: The histopathologic studies no recorded neoplasias, only mild changes and no significant alterations. The hemo-oximetry (carboxyhemoglobin and methemoglobim) and CO2 concentration confirm that the animals were submitted to high intensity of exposure to carcinogens in tobacco and its derivatives. CONCLUSION: The experimental models were highly efficient, practical, easy to use and economical and can be employed in other similar studies to determine the harmful effects by smoking and reflux.
Subject: Smoking
Hydrochloric Acid
Pepsin
Gastroesophageal Reflux
Rats
Country: Brazil
Editor: Acta Cirurgica Brasileira
Rights: aberto
Date Issue: 2012
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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