Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/1835
Type: Artigo de periódico
Title: Polyvinyl alcohol associated with carbon nanotube scaffolds for osteogenic differentiation of rat bone mesenchymal stem cells
Author: Rodrigues, Ana A.
Batista, Nilza A.
Bavaresco, Vanessa P.
Baranauskas, Vitor
Ceragioli, Helder J.
Peterlevitz, Alfredo C.
Santos, Arnaldo R., Jr.
Belangero, William D.
Abstract: Polyvinyl alcohol (PVAl) hydrogel, alone and reinforced with two types of carbon nanopartides, was studied in cultured cells to assess its potential use in treating osteochondral defects. The carbon nanoparticles were produced by hot-filament chemical vapour deposition. The carbon material was characterised with a Renishaw Invia Raman microscope system and the morphological particles were characterised with field emission scanning electron microscopy and high-resolution transmission electron microscopy. Cytotoxicity was evaluated by measuring the Vero fibroblast-type cells' metabolic activity and studying their morphology. The osteogenic differentiation of mesenchymal stem cells obtained from rat bone marrow was evaluated by alkaline phosphatase (ALP) and alizarin red S (ARS) staining. Cell viability and morphology were assessed with thiazolyl blue tetrazolium bromide and scanning electron microscopy, respectively. The materials did not interfere with the viability, metabolic activity, morphology and spreading of either of the cell types analysed. Nodules of mineralised organic matrix were identified with ARS and ALP, confirming osteogenic differentiation. These results indicated higher concentration of ALP and mineralised matrix for PVAl with carbon nanoparticles. The results of this study indicate the potential use of carbon nanoparticles with PVAl hydrogels as orthopaedic biomaterials to treat osteochondral defects, but further in vivo investigations are still necessary. (C) 2011 Elsevier Ltd. All rights reserved.
Editor: Pergamon-Elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.carbon.2011.08.071
Date Issue: 2012
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.