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Type: Artigo de periódico
Title: Sildenafil preserves diastolic relaxation after reduction by L-NAME and increases phosphodiesterase-5 in the intercalated discs of cardiac myocytes and arterioles
Author: FERREIRA-MELO, Silvia Elaine
DEMACQ, Caroline
KRIEGER, José Eduardo
IRIGOYEN, Maria Cláudia
MORENO, Heitor
Abstract: OBJECTIVES: We investigated the influence of sildenafil on cardiac contractility and diastolic relaxation and examined the distribution of phosphodiesterase-5 in the hearts of hypertensive rats that were treated with by NG-nitro-L-arginine methyl ester (L-NAME). METHODS: Male Wistar rats were treated with L-NAME and/or sildenafil for eight weeks. The Langendorff method was used to examine the effects of sildenafil on cardiac contractility and diastolic relaxation. The presence and location of phosphodiesterase-5 and phosphodiesterase-3 were assessed by immunohistochemistry, and cGMP plasma levels were measured by ELISA. RESULTS: In isolated hearts, sildenafil prevented the reduction of diastolic relaxation (dP/dt) that was induced by L-NAME. In addition, phosphodiesterase-5 immunoreactivity was localized in the intercalated discs between the myocardial cells. The staining intensity was reduced by L-NAME, and sildenafil treatment abolished this reduction. Consistent with these results, the plasma levels of cGMP were decreased in the L-NAME-treated rats but not in rats that were treated with L-NAME + sildenafil. CONCLUSION: The sildenafil-induced attenuation of the deleterious hemodynamic and cardiac morphological effects of L-NAME in cardiac myocytes is mediated (at least in part) by the inhibition of phosphodiesterase-5.
Subject: Hypertension
Phosphodiesterase-5 Inhibitor
Country: Brasil
Editor: Faculdade de Medicina / USP
Citation: Clinics, v.66, n.7, p.1253-1258, 2011
Rights: aberto
Identifier DOI: 10.1590/S1807-59322011000700022
Date Issue: 2011
Appears in Collections:FCM - Artigos e Outros Documentos

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