Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/12596
Type: Artigo de periódico
Title: Caracterização do material de reserva em feijão-guandu, lablabe e mucuna
Title Alternative: Characterization of the storage material in guandu, lab-lab and mucuna
Author: Seno, Maria Silvia
Gomes, Laurecir
Cortelazzo, Angelo Luiz
Abstract: For the cytochemical characterization of the storage material present in guandu, lab-lab and mucuna, seeds of each species were fixed and prepared for microscopy. Routine and specific fixation methods for proteins, lipids, neutral and acidic polysaccharides were used. The main reserves of the seeds were also analyzed and characterized. The results showed that starch is the principal reserve, with proteins surrounding the starch grains, as well as a few lipid globules. The starch concentration was 55% of the dry weight (DW) for guandu, 53% for lab-lab and 40% for mucuna. The proteins were more abundant in lab-lab, with 26% DW, while guandu and mucuna had respectively 19% and 17%. Lipids represented up to 2% of the total DW of the three species with mucuna presenting the largest quantity. The analyses of neutral saccharides showed a large amount of glucose due to the starch reserve, but also appreciable quantities of galactose and arabinose, which always occurred in greater quantities in mucuna. Electrophoretic patterns for proteins of guandu and lab-lab were quite similar, suggesting a a phylogenetic proximity between these species. The principal proteins had a molecular relative mass between 65 to 70 kg/mol.
Com o intuito de caracterizar, em nível citoquímico, as principais reservas presentes em guandu, lablabe e mucuna, sementes foram fixadas e processadas para a microscopia, utilizando-se tanto métodos gerais como específicos para proteínas, lipídios, polissacarídeos neutros e ácidos, procedendo-se, à dosagem e à caracterização dessas reservas nas sementes. O amido revelou-se como principal material, seguido por proteínas e, em menor grau, por lipídios. O conteúdo de amido foi de 55% da matéria seca (MS) para guandu, 53% para lablabe e 40% para mucuna. As proteínas foram mais abundantes em lablabe, com 26% da MS, seguindo-se o guandu (19%) e a mucuna (17%). Lipídios representaram menos de 2% da MS dessas sementes, com uma quantidade maior em mucuna do que nas demais espécies. As análises dos açúcares neutros presentes indicaram grande conteúdo de glicose em função do amido e quantidade apreciável de galactose e arabinose, sempre em maior quantidade em mucuna. Os perfis eletroforéticos revelaram semelhança maior entre as proteínas de guandu e lablabe, sugerindo maior proximidade filogenética entre tais espécies. As proteínas mais abundantes apresentaram massa molecular relativa entre 65 e 70 kg/mol.
Subject: leguminosas
sementes
Cajanus cajan
Lab-lab purpureum
Mucuna aterrima
reservas
leguminous
seeds
Cajanus cajan
Lab-lab purpureum
Mucuna aterrima
storage
Editor: Instituto Agronômico de Campinas
Rights: aberto
Identifier DOI: 10.1590/S0006-87051996000100006
Address: http://dx.doi.org/10.1590/S0006-87051996000100006
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051996000100006
Date Issue: 1-Jan-1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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