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Type: Artigo de periódico
Title: Proteome analysis of human dorsolateral prefrontal cortex using shotgun mass spectrometry
Author: MARTINS-DE-SOUZA, Daniel
GATTAZ, Wagner F.
REWERTS, Christiane
MACCARRONE, Giuseppina
DIAS-NETO, Emmanuel
TURCK, Christoph W.
Abstract: The prefrontal cortex executes important functions such as differentiation of conflicting thoughts, correct social behavior and personality expression, and is directly implicated in different neurodegenerative diseases. We performed a shotgun proteome analysis that included IEF fractionation, RP-LC, and MALDI-TOF/TOF mass spectrometric analysis of tryptic digests from a pool of seven human dorsolateral prefrontal cortex protein extracts. In this report, we present a catalog of 387 proteins expressed in these samples, identified by two or more peptides and high confidence search scores. These proteins are involved in different biological processes such as cell growth and/or maintenance, metabolism/energy pathways, cell communication/signal trarisduction, protein metabolism, transport, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism, and immune response. This analysis contributes to the knowledge of the human brain proteome by adding sample diversity and protein expression data from an alternative technical approach. It will also aid comparative studies of different brain areas and medical conditions, with future applications in basic and clinical research.
Subject: brain
mass spectrometry
prefrontal cortex
shotgun proteomics
Country: Alemanha
Citation: JOURNAL OF SEPARATION SCIENCE, v.31, n.16-17, p.3122-3126, 2008
Rights: fechado
Identifier DOI: 10.1002/jssc.200800224
Date Issue: 2008
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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