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Type: Artigo de periódico
Title: Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9
Author: DEMARCHI, Ana Claudia Cardoso de Oliveira
ZAMBUZZI, Willian Fernando
PAIVA, Katiucia Batista Silva
SILVA-VALENZUELA, Maria das Gracas da
NUNES, Fabio Daumas
FIGUEIRA, Rita de Cassia Savio
SASAHARA, Regina Maki
DEMASI, Marcos Angelo Almeida
WINNISCHOFER, Sheila Maria Brochado
SOGAYAR, Mari Cleide
Abstract: We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner.
Subject: RECK
Palate development
ECM remodeling
Country: Estados Unidos
Citation: CELL AND TISSUE RESEARCH, v.340, n.1, p.61-69, 2010
Rights: fechado
Identifier DOI: 10.1007/s00441-010-0931-6
Date Issue: 2010
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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