Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/1116
Type: Artigo de periódico
Title: Mechanism of Trypanosoma cruzi death induced by Cratylia mollis seed lectin
Author: FERNANDES, M. P.
INADA, N. M.
CHIARATTI, M. R.
ARAUJO, F. F. B.
MEIRELLES, F. V.
CORREIA, M. T. S.
COELHO, L. C. B. B.
ALVES, M. J. M.
GADELHA, F. R.
VERCESI, A. E.
Abstract: Incubation of T. cruzi epimastigotes with the lectin Cramoll 1,4 in Ca(2+) containing medium led to agglutination and inhibition of cell proliferation. The lectin (50 A mu g/ml) induced plasma membrane permeabilization followed by Ca(2+) influx and mitochondrial Ca(2+) accumulation, a result that resembles the classical effect of digitonin. Cramoll 1,4 stimulated (five-fold) mitochondrial reactive oxygen species (ROS) production, significantly decreased the electrical mitochondrial membrane potential (Delta I(m)) and impaired ADP phosphorylation. The rate of uncoupled respiration in epimastigotes was not affected by Cramoll 1,4 plus Ca(2+) treatment, but oligomycin-induced resting respiration was 65% higher in treated cells than in controls. Experiments using T. cruzi mitochondrial fractions showed that, in contrast to digitonin, the lectin significantly decreased Delta I(m) by a mechanism sensitive to EGTA. In agreement with the results showing plasma membrane permeabilization and impairment of oxidative phosphorylation by the lectin, fluorescence microscopy experiments using propidium iodide revealed that Cramoll 1,4 induced epimastigotes death by necrosis.
Subject: Trypanosoma cruzi
Mitochondria
Calcium
ROS
Cell death
Cramoll 1,4 lectin
Country: Estados Unidos
Editor: SPRINGER/PLENUM PUBLISHERS
Citation: JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, v.42, n.1, p.69-78, 2010
Rights: fechado
Identifier DOI: 10.1007/s10863-010-9268-9
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000275782100008&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1007/s10863-010-9268-9
Date Issue: 2010
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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