Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/1094
Type: Artigo de periódico
Title: Recovery of DNA for the detection and genotyping of human papillomavirus from clinical cervical specimens stored for up to 2 years in a universal collection medium with denaturing reagent
Author: CAMPOS, Elisabete A.
SIMOES, Jose Antonio
RABELO-SANTOS, Silvia H.
SARIAN, Luis Otavio
PITTA, Denise Rocha
LEVI, Jose Eduardo
DERCHAIN, Sophie
Abstract: The recovery and stability of DNA for the detection and genotyping of HPV in UCM-containing specimens, after exposure to denaturing reagents and stored for up to 2 years were evaluated. Samples were collected from 60 women who had cervical cytology specimens harboring cervical intraepithelial neoplasia (CIN) 2 or 3. All samples were stored in UCM and had been frozen at -20 degrees C following the addition of the denaturing reagent (sodium hydroxide) and the removal of the aliquot required for Hybrid Capture 2 testing for the identification of HPV DNA. The samples had been stored for 6, 12 and 24 months (20 samples for each storage time). HPV DNA extraction was performed according to a protocol designed specifically and the presence and quality of DNA was confirmed by human P-globin detection using the consensus primers G73 and G74. HPV DNA was amplified using the consensus primers PGMY09 and PGMY11, and reverse line-blot hybridization was used to detect type-specific amplicons for 37 HPV types. The DNA extracted from the denatured specimen was recovered in 57/60 (95%) of the samples. HPV DNA was detected in 56/57 (98%) of the recovered samples. Twenty-six of the 56 samples recovered (48%) were genotyped successfully. (c) 2007 Elsevier B.V. All rights reserved.
Subject: HPV DNA
universal collection medium
denaturing reagent
detection
storage
cervical cancer
genotype
Country: Holanda
Editor: ELSEVIER SCIENCE BV
Rights: fechado
Identifier DOI: 10.1016/j.jviromet.2007.09.014
Address: http://dx.doi.org/10.1016/j.jviromet.2007.09.014
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Date Issue: 2008
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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