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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAlvarenga, Elenice Montept_BR
dc.contributor.authorunicampMartins, James Alexandrept_BR
dc.contributor.authorunicampVidal, Benedicto de Campospt_BR
dc.contributor.authorunicampRincones, Johanapt_BR
dc.contributor.authorunicampCarazzolle, Marcelo Falsarellapt_BR
dc.contributor.authorunicampMello, Maria Luiza Silveirapt_BR
dc.typeArtigopt_BR
dc.titleSpatial distribution of AT- and GC-rich DNA within interphase cell nuclei of Triatoma infestans Klugpt_BR
dc.contributor.authorAlvarenga, Elenice M.pt_BR
dc.contributor.authorMondin, Mateuspt_BR
dc.contributor.authorMartins, James A.pt_BR
dc.contributor.authorRodrigues, Vera L.C.C.pt_BR
dc.contributor.authorVidal, Benedicto C.pt_BR
dc.contributor.authorRincones, Johanapt_BR
dc.contributor.authorCarazzolle, Marcelo F.pt_BR
dc.contributor.authorAndrade, Larissa Marapt_BR
dc.contributor.authorMello, Maria Luiza S.pt_BR
unicamp.authorAlvarenga, E.M., Department of Anatomy, Cell Biology and Physiology, Institute of Biology, University of Campinas (UNICAMP), 13083-863 Campinas, SP, Brazilpt_BR
unicamp.authorMartins, J.A., Department of Anatomy, Cell Biology and Physiology, Institute of Biology, University of Campinas (UNICAMP), 13083-863 Campinas, SP, Brazilpt_BR
unicamp.authorVidal, B.C., Department of Anatomy, Cell Biology and Physiology, Institute of Biology, University of Campinas (UNICAMP), 13083-863 Campinas, SP, Brazilpt_BR
unicamp.authorRincones, J., Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazilpt_BR
unicamp.authorCarazzolle, M.F., Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazilpt_BR
unicamp.authorMello, M.L.S., Department of Anatomy, Cell Biology and Physiology, Institute of Biology, University of Campinas (UNICAMP), 13083-863 Campinas, SP, Brazilpt_BR
unicamp.author.externalMondin, M., Department of Genetics, Luiz de Queiroz College of Agriculture-ESALQ, University of São Paulo (USP), 13418-900 Piracicaba, SP, Brazilpt
unicamp.author.externalRodrigues, V.L.C.C., SUCEN, 13840-000 Mogi-Guaçu, SP, Brazilpt
unicamp.author.externalAndrade, L.M., Department of Genetics, Luiz de Queiroz College of Agriculture-ESALQ, University of São Paulo (USP), 13418-900 Piracicaba, SP, Brazilpt
dc.subjectTriatoma infestanspt_BR
dc.subjectHeterocromatinapt_BR
dc.subjectDNApt_BR
dc.subjectEpigenéticapt_BR
dc.subjectBioinformáticapt_BR
dc.subjectMicroscopiapt_BR
dc.subject.otherlanguageTriatoma infestanspt_BR
dc.subject.otherlanguageHeterochromatinpt_BR
dc.subject.otherlanguageDNApt_BR
dc.subject.otherlanguageEpigeneticspt_BR
dc.subject.otherlanguageBioinformaticspt_BR
dc.subject.otherlanguageMicroscopypt_BR
dc.description.abstractHeterochromatin bodies in single- and multichromocentered interphase cell nuclei of Triatoma infestans, a vector of Chagas disease, have been suggested to contain AT-rich DNA, based on their positive response to Q-banding and Hoechst 33248 treatment. No information exists on whether GC-rich DNA is also present in these nuclei and whether it plays a role on chromatin condensation. Considering that methodologies more precise than those previously used to determine DNA base composition in situ are currently available, and that the spatial distribution of chromatin areas differing in composition in interphase cell nuclei of different species is a matter of interest, the localization of AT- and GC-rich DNA in T. infestans nuclei is revisited here. The methodologies used included DAPI/AMD and CMA3/Distamycin differential staining, Feulgen-DNA image analysis following Msp I and Hpa II enzymatic digestion, 5-methylcytidine immunodetection, AgNOR response, confocal microscopy, and the 5-aza-2'-deoxycytidine (5-AZA) demethylation assay. The results identified the presence of AT-rich/GC-poor DNA in chromocenters and evenly distributed AT and GC sequences in euchromatin. A GC-rich DNA zone encircling the chromocenters was also found but it could not be associated with NOR regions. To corroborate the DNA AT-richness in T. infestans nuclei, bioinformatic analyses were also performed. Methylated cytosine was evident at some points of the chromocenters' edge in single- and multichromocentered nuclei and at the euchromatin of multichromocentered nuclei and could be transiently affected by the 5-AZA treatment. The present results suggest that in the particular case of chromocenters of the hemipteran T. infestans, cytosine methylation is not a relevant factor involved in chromatin condensation. © 2011 Elsevier Ltd.en
dc.description.abstractHeterochromatin bodies in single- and multichromocentered interphase cell nuclei of Triatoma infestans, a vector of Chagas disease, have been suggested to contain AT-rich DNA, based on their positive response to Q-banding and Hoechst 33248 treatment. No information exists on whether GC-rich DNA is also present in these nuclei and whether it plays a role on chromatin condensation. Considering that methodologies more precise than those previously used to determine DNA base composition in situ are currently available, and that the spatial distribution of chromatin areas differing in composition in interphase cell nuclei of different species is a matter of interest, the localization of AT- and GC-rich DNA in T. infestans nuclei is revisited here. The methodologies used included DAPI/AMD and CMA3/Distamycin differential staining, Feulgen-DNA image analysis following Msp I and Hpa II enzymatic digestion, 5-methylcytidine immunodetection, AgNOR response, confocal microscopy, and the 5-aza-2'-deoxycytidine (5-AZA) demethylation assay. The results identified the presence of AT-rich/GC-poor DNA in chromocenters and evenly distributed AT and GC sequences in euchromatin. A GC-rich DNA zone encircling the chromocenters was also found but it could not be associated with NOR regions. To corroborate the DNA AT-richness in T. infestans nuclei, bioinformatic analyses were also performed. Methylated cytosine was evident at some points of the chromocenters' edge in single- and multichromocentered nuclei and at the euchromatin of multichromocentered nuclei and could be transiently affected by the 5-AZA treatment. The present results suggest that in the particular case of chromocenters of the hemipteran T. infestans, cytosine methylation is not a relevant factor involved in chromatin condensation.pt
dc.relation.ispartofMicron: the international research and review journal for microscopypt_BR
dc.relation.ispartofabbreviationMicronpt_BR
dc.publisher.cityNew York, NYpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherPergamon Presspt_BR
dc.date.issued2011pt_BR
dc.date.monthofcirculationaug.pt_BR
dc.identifier.citationMicron. , v. 42, n. 6, p. 568 - 578, 2011.pt_BR
dc.language.isoengpt_BR
dc.description.volume42pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage568pt_BR
dc.description.lastpage578pt_BR
dc.rightsfechadopt_BR
dc.sourceScopuspt_BR
dc.sourceSCOPUSpt_br
dc.identifier.issn0968-4328pt_BR
dc.identifier.eissn1878-4291pt_BR
dc.identifier.doi10.1016/j.micron.2011.02.002pt_BR
dc.identifier.urlhttps://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0968432811000230pt_BR
dc.description.sponsorshipCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipFAPESP – FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipCNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1CNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1FAPESP – FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1CNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1CNPQ – CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumber47303/2009-7pt_BR
dc.description.sponsordocumentnumber2010-50015-6pt_BR
dc.description.sponsordocumentnumber132341/2010-7pt_BR
dc.description.sponsordocumentnumber503702/2007-2pt_BR
dc.description.sponsordocumentnumber301943/2009-5pt_BR
dc.date.available2015-06-30T20:41:56Z-
dc.date.available2015-11-26T14:53:50Z-
dc.date.accessioned2015-06-30T20:41:56Z-
dc.date.accessioned2015-11-26T14:53:50Z-
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dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/108928-
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/108928-
dc.identifier.idScopus2-s2.0-79955634542pt_BR
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dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source2-s2.0-79955634542-
dc.creator.orcid0000-0003-0285-1653pt_BR
dc.creator.orcid0000-0002-5474-2830pt_BR
dc.creator.orcid0000-0003-1629-5619pt_BR
dc.type.formArtigo original-
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