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|Type:||Artigo de periódico|
|Title:||Improvement Of Phytase Activity By A New Saccharomyces Cerevisiae Strain Using Statistical Optimization|
Alves MacEdo G.
|Abstract:||Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2 2 full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae. © 2011 Edi Franciele Ries and Gabriela Alves Macedo.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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