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Type: Artigo de periódico
Title: Use Of Agroindustrial Residues In Biotechnological Process By Betagalactosidase Production From Kluyveromyces Marxianus Cct 7082 [utilização De Resíduos Agroindustriais Em Processo Biotecnológico Para Produção De β-galactosidase De Kluyveromyces Marxianus Cct 7082]
Author: Manera A.P.
Ores J.C.
Ribeiro V.A.
Rodrigues M.I.
Kalil S.J.
Filho F.M.
Abstract: The objective of this work was to evaluate the composition of the culture medium for the production of the enzyme β-galactosidase from Kluyveromyces marxianus CCT 7082 using an experimental design. Enzyme production was carried out in a medium consisting of cheese whey, corn steep liquor (CSL) and Prodex-lac® yeast hydrolyzate. A factorial fractional design (25-1) was performed to determine the significant variables in the production of the enzyme. Different concentrations of lactose present in whey (10-70 g L-1), CSL (10-100 g L-1), Prodex-lac (40- 20 g L-1), (NH4)2SO4 (0-10 g L-1) and pH (5-7) were tested. Lactose concentration, CSL and pH were the variables that showed a statistically significant effect on enzyme activity within the ranges studied, and these variables were used in a central composite rotatable design to optimize production of the enzyme. The ranges used were: lactose concentration 40-100 g L-1, CSL 10-120 g L-1, and pH 3.5-6.5. The conditions that resulted in higher enzyme activity (1400 U g-1) were 70 g L-1 lactose, 65 g L-1 CSL, 4 g L-1 Prodex-lac and pH 5, yielding 61 U L-1 h-1.
Rights: aberto
Identifier DOI: 10.4025/actascitechnol.v33i2.9919
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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