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Type: Artigo de periódico
Title: Two-dimensional Difference Gel Electrophoresis Applied For Analytical Proteomics: Fundamentals And Applications To The Study Of Plant Proteomics
Author: Arruda S.C.C.
Barbosa H.D.S.
Azevedo R.A.
Arruda M.A.Z.
Abstract: The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical applications of this technique are pointed out, emphasizing its potentialities in plant proteomics. © 2011 The Royal Society of Chemistry.
Rights: aberto
Identifier DOI: 10.1039/c1an15513j
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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