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|Type:||Artigo de periódico|
|Title:||Two-dimensional Difference Gel Electrophoresis Applied For Analytical Proteomics: Fundamentals And Applications To The Study Of Plant Proteomics|
|Abstract:||The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical applications of this technique are pointed out, emphasizing its potentialities in plant proteomics. © 2011 The Royal Society of Chemistry.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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