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Type: Artigo de periódico
Title: New Aqueous Two-phase System Based On Cashew-nut Tree Gum And Poly(ethylene Glycol)
Author: Sarubbo L.A.
Oliveira L.A.
Porto A.L.F.
Duarte H.S.
Carneiro-Leao A.M.A.
Lima-Filho J.L.
Campos-Takaki G.M.
Tambourgi E.B.
Abstract: The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification. (C) 2000 Elsevier Science B.V.
Rights: fechado
Identifier DOI: 10.1016/S0378-4347(99)00516-2
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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