Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Characterization Of The Intracellular Ca2+ Pools Involved In The Calcium Homeostasis In Herpetomonas Sp. Promastigotes
Author: Sodre C.L.
Moreira B.L.M.
Nobrega F.B.
Gadelha F.R.
Meyer-Fernandes J.R.
Dutra P.M.L.
Vercesi A.E.
Lopes A.H.C.S.
Scofano H.M.
Barrabin H.
Abstract: Trypanosomatids of the genus Herpetomonas comprises monoxenic parasites of insects that present proand opisthomastigotes forms in their life cycles. In this study, we investigated the Ca2+ transport and the mitochondrial bioenergetic of digitonin-permeabilized Herpetomonas sp. promastigotes. The response of promastigotes mitochondrial membrane potential to ADP, oligomycin, Ca2+, and antimycin A indicates that these mitochondria behave similarly to vertebrate and Trypanosoma cruzi mitochondria regarding the properties of their electrochemical proton gradient. Ca2+ transport by permeabilized cells appears to be performed mainly by the mitochondria. Unlike T. cruzi, it was not possible to observe Ca2+ release from Herpetomonas sp. mitochondria, probably due to the simultaneous Ca2+ uptake by the endoplasmic reticulum. In addition, a vanadate-sensitive Ca2+ transport system, attributed to the endoplasmic reticulum, was also detected. Nigericin (1 μM), FCCP (1 μM), or bafilomycin A1 (5 μM) had no effect on the vanadate-sensitive Ca2+ transport. These data suggest the absence of a Ca2+ transport mediated by a Ca2+/H+ antiport. No evidence of a third Ca2+ compartment with the characteristics of the acidocalcisomes described by A. E. Vercesi et al. (1994, Biochem. J. 304, 227-233) was observed. Thapsigargin and IP3 were not able to affect the vanadate-sensitive Ca2+ transport. Ruthenium red was able to inhibit the Ca2+ uniport of mitochondria, inducing a slow mitochondrial Ca2+ efflux, compatible with the presence of a Ca2+/H+ antiport. Moreover, this efflux was not stimulated by the addition of NaCl, which suggests the absence of a Ca2+/Na+ antiport in mitochondria. (C) 2000 Academic Press.
Rights: fechado
Identifier DOI: 10.1006/abbi.2000.1899
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
2-s2.0-0034254234.pdf108.27 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.