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|Type:||Artigo de periódico|
|Title:||Characterization Of Monoterpene Biotransformation In Two Pseudomonads|
|Abstract:||Aims: To study the metabolic profile of Pseudomonas rhodesiae and Pseudomonas fluorescens in water-organic solvent systems using terpene substrates for both growth and biotransformation processes and to determine the aerobic or anaerobic status of these degradation pathways. Materials and Methods: Substrates from pinene (α-pinene, α-pinene oxide, β-pinene, β-pinene oxide, turpentine) and limonene (limonene, limonene-1,2-oxide, orange peel oil) families were tested. For the bioconversion, the terpene-grown biomass was concentrated and used either as whole cells or as a crude enzymatic extract. Conclusion: Pseudomonas rhodesiae was the most suitable biocatalyst for the production of isonovalal from α-pinene oxide and did not metabolize limonene. Pseudomonas fluorescens was a more versatile micro-organism and metabolized limonene in two different ways. The first (anaerobic, cofactor-independent, noninducible) allowed limonene elimination by synthesizing α-terpineol. The second (aerobic, cofactor-dependent) involved limonene-1,2-oxide as an intermediate for energy production through a β-oxidation process. Significance and Impact of the Study: Enzymatic isomerization of β- to α-pinene was described for the first time for both strains. Alpha-terpineol production by P. fluorescens was very efficient and appeared as a promising alternative for the commercial production of this bioflavour. © 2008 The Authors.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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