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|Type:||Artigo de periódico|
|Title:||Kinectic Characterization And Flavonoid Effect On Human Lymphocyte Protein Tyrosyne Phosphatase|
|Abstract:||The aim of this work was to determine the kinetic properties and evaluate the effect of some flavonoids oh human lymphocyte protein tyrosine phosphatase. Tyrosine-, serine- and threonine-phosphate were hydrolyzed by this phosphatase 60%, 20% and 10%, respectively. In the kinetic studies the enzymatic activity was determined by using p-nitrophenylphosphate (pNPP) as substrate. The enzyme presented optimum pH around 5.0 and was inhibited by 100 μM p-chloromercuribenzoate (pCMB) (80%), 10 mM fluoride (35%), 10 mM vanadate (100%), and 5mM Cu +2 (85%). The enzyme was also strongly inhibited by a tyrosine phosphatase inhibitor cocktail, but was unaffected by okadaic acid. These results confirm that the major phosphatase activity in human lymphocytes is a protein tyrosine phosphatase. Among the bioflavonoids tested only fisetin showed an inhibitory effect in order of 80% on the enzymatic activity.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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