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Type: Artigo de periódico
Title: Kinectic Characterization And Flavonoid Effect On Human Lymphocyte Protein Tyrosyne Phosphatase
Author: Lemos T.M.A.M.
Miranda M.A.
Cavagis A.D.M.
Aoyama H.
Ferreira C.V.
Abstract: The aim of this work was to determine the kinetic properties and evaluate the effect of some flavonoids oh human lymphocyte protein tyrosine phosphatase. Tyrosine-, serine- and threonine-phosphate were hydrolyzed by this phosphatase 60%, 20% and 10%, respectively. In the kinetic studies the enzymatic activity was determined by using p-nitrophenylphosphate (pNPP) as substrate. The enzyme presented optimum pH around 5.0 and was inhibited by 100 μM p-chloromercuribenzoate (pCMB) (80%), 10 mM fluoride (35%), 10 mM vanadate (100%), and 5mM Cu +2 (85%). The enzyme was also strongly inhibited by a tyrosine phosphatase inhibitor cocktail, but was unaffected by okadaic acid. These results confirm that the major phosphatase activity in human lymphocytes is a protein tyrosine phosphatase. Among the bioflavonoids tested only fisetin showed an inhibitory effect in order of 80% on the enzymatic activity.
Rights: fechado
Identifier DOI: 
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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